6YM9
Mycobacterium tuberculosis FtsZ in complex with GTP-gamma-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 86 |
Detector technology | PIXEL |
Collection date | 2019-07-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 89.063, 89.063, 181.327 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.130 - 2.030 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rq7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.143 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 181.330 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 51881 | 7482 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 99.0 | |
Redundancy | 5.7 | |
CC(1/2) | 0.998 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 291.15 | 27.5% PEG4000, 0.4 M ammonium acetate and 0.1 M Na citrate, soaked with 10 mM GPS for 4 hr. |