6YLQ

EGFP in neutral pH, Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	50.940, 62.120, 68.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.390 - 1.650
R-factor	0.1801
Rwork	0.178
R-free	0.21650
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	1.803
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.390	39.390	1.670
High resolution limit [Å]	1.630	7.290	1.630
Rmerge	0.077	0.032	1.623
Rmeas	0.084	0.034	2.078
Total number of observations	143428
Number of reflections	24211	352	644
<I/σ(I)>	15.35	55.09	0.55
Completeness [%]	86.5	96.4	31.6
Redundancy	5.924	7.29	1.739
CC(1/2)	0.999	0.999	0.285

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 8000, HEPES, magnesium chloride