6YLP
EGFP_in_Acidic_env Directionality of Optical Properties of Fluorescent Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-13 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.5419 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.970, 62.150, 68.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.120 - 1.550 |
R-factor | 0.1776 |
Rwork | 0.176 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y0g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.720 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.120 | 46.120 | 1.590 |
High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
Rmerge | 0.055 | 0.029 | 0.894 |
Rmeas | 0.060 | 0.031 | 1.157 |
Total number of observations | 353489 | ||
Number of reflections | 61255 | 680 | 4560 |
<I/σ(I)> | 16.94 | 65.52 | 1.03 |
Completeness [%] | 99.9 | 99.3 | 99.9 |
Redundancy | 5.771 | 8.941 | 2.49 |
CC(1/2) | 0.999 | 1.000 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 298 | PEG 8000, potassium dihydrogen phosphate |