6YLN
mTurquoise2 SG P212121 - Directional optical properties of fluorescent proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-03 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.54187 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.170, 61.270, 68.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.270 - 1.850 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ztf |
RMSD bond length | 0.016 |
RMSD bond angle | 1.711 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.270 | 39.270 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.052 | 0.023 | 1.906 |
Rmeas | 0.053 | 0.024 | 2.058 |
Total number of observations | 239608 | ||
Number of reflections | 18997 | 248 | 1375 |
<I/σ(I)> | 29.51 | 128.93 | 0.95 |
Completeness [%] | 99.9 | 97.6 | 99.2 |
Redundancy | 12.613 | 19.835 | 6.809 |
CC(1/2) | 1.000 | 1.000 | 0.496 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 8000, HEPES, Magnesium chloride |