6YL9
Crystal structure of YTHDC1 with compound DHU_DC1_085
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.740, 103.200, 42.400 |
Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
Resolution | 41.000 - 1.500 |
R-factor | 0.1719 |
Rwork | 0.171 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 52073 | 8384 |
<I/σ(I)> | 27.9 | |
Completeness [%] | 97.6 | |
Redundancy | 3.25 | |
CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |