6YL6

Cdk2(F80C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	298
Detector technology	CCD
Collection date	2017-02-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	52.654, 71.357, 71.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.410 - 1.700
Rwork	0.181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ek3
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.410	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	29979	2672
<I/σ(I)>	16.92
Completeness [%]	98.9
Redundancy	2
CC(1/2)	1.000	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Protein solution: 8 mg/mL, 100 mM phosphate buffer (pH6.2) Reservoir solution: 8-12% PEG3350, HEPES (0.1 M pH 7.5 - 8.5)