6YJJ

Structure of CYRI-B (FAM49B) from Rhincodon typus (selenomethionine derivative)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9611
Spacegroup name	P 21 21 21
Unit cell lengths	40.400, 72.820, 107.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.660 - 2.400
R-factor	0.2067
Rwork	0.205
R-free	0.24150
Structure solution method	SAD
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.820	72.820	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.123	0.052	1.032
Rmeas	0.130	0.055	1.102
Rpim	0.042	0.018	0.382
Total number of observations	123774	2485	10889
Number of reflections	13006	308	1329
<I/σ(I)>	12.8	36.7	2.1
Completeness [%]	100.0	99.9	100
Redundancy	9.5	8.1	8.2
CC(1/2)	0.998	0.997	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	25% PEG3350, 0.1 M Bis-Tris pH 5.5 and 0.2 M ammonium acetate