6YJI
Structure of FgCelDH7C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976253 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.536, 187.821, 55.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.180 - 1.640 |
| R-factor | 0.1656 |
| Rwork | 0.164 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k8e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.180 | 1.699 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.183 | 1.547 |
| Rpim | 0.052 | 0.504 |
| Number of reflections | 119235 | 9035 |
| <I/σ(I)> | 6.22 | 1.07 |
| Completeness [%] | 95.5 | 73.16 |
| Redundancy | 13 | 10.1 |
| CC(1/2) | 0.996 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | PEG 3350 20% (w/v) Sodium thiocyanate 100 mM |
