6YJA
DarB fom B. subtilis in complex with c-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.150, 65.410, 104.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.910 - 1.700 |
R-factor | 0.1842 |
Rwork | 0.183 |
R-free | 0.21050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yav |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.910 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 17.000 | 1.700 |
Rmerge | 0.048 | 0.038 | 0.855 |
Rmeas | 0.052 | 0.043 | 0.920 |
Total number of observations | 231089 | ||
Number of reflections | 32669 | 45 | 3231 |
<I/σ(I)> | 24.45 | 43.62 | 2.41 |
Completeness [%] | 99.8 | 93.8 | 99.8 |
Redundancy | 7.074 | 4.356 | 7.34 |
CC(1/2) | 0.999 | 0.996 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2 M calcium chloride dehydrate, 0.05 M HEPES sodium pH 7.5, 28 % v/v polyethylene glycol 400, 0.002 M spermine |