6YJ9

DarB in complex with 3'3'cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	41.490, 69.920, 105.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.680 - 1.500
R-factor	0.1551
Rwork	0.153
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yav
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.680	50.000	1.520
High resolution limit [Å]	1.500	17.000	1.500
Rmerge	0.037	0.022	0.608
Rmeas	0.039	0.024	0.632
Total number of observations	650885
Number of reflections	50229	45	1915
<I/σ(I)>	31.06	53.68	4.2
Completeness [%]	99.9	84.9	100
Redundancy	12.958	5.556	13.341
CC(1/2)	1.000	1.000	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1 M Tris/HCl PH 8.5, 32 % w/v polyethylene glycol 4000, 5 % v/v/ glycerol