6YJ1
The M23 peptidase domain of the Staphylococcal phage 2638A endolysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.890, 56.380, 110.490 |
| Unit cell angles | 90.00, 92.84, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.300 |
| R-factor | 0.2543 |
| Rwork | 0.251 |
| R-free | 0.30960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.074 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.161 | 0.533 |
| Rmeas | 0.176 | 0.585 |
| Rpim | 0.070 | 0.235 |
| Number of reflections | 17866 | 1664 |
| <I/σ(I)> | 7.59 | 4.1 |
| Completeness [%] | 95.1 | 89.46 |
| Redundancy | 6.01 | 5.74 |
| CC(1/2) | 0.986 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | Protein buffer: 20 mM Tris, 150 mM NaCl, pH 7.4 Crystallization buffer: 0.2 M sodium chloride, 30 % (v/v) PEG 300, pH 5.7 |
