6YIG
Crystal structure of the N-terminal EF-hand domain of Arabidopsis thaliana AtEH1/Pan1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.510, 38.620, 63.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 1.550 |
R-factor | 0.1459 |
Rwork | 0.145 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qpt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.931 |
Data reduction software | XDS (20180409) |
Data scaling software | XSCALE (20180409) |
Phasing software | MoRDa |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.000 | 33.000 | 1.590 |
High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
Rmeas | 0.052 | 0.029 | 0.387 |
Number of reflections | 23759 | 273 | 1451 |
<I/σ(I)> | 22.88 | 63.43 | 3.4 |
Completeness [%] | 97.2 | 100 | 81.4 |
Redundancy | 7.47 | 8.74 | 3.64 |
CC(1/2) | 0.999 | 0.999 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 100mM HEPES pH 7.6, 0.8M SodiumMalonate, 0.5% Jeffamine |