6YHV
Structural insights into Pseudomonas aeruginosa Type six secretion system exported effector 8: unliganded Tse8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 |
Unit cell lengths | 66.506, 68.078, 77.604 |
Unit cell angles | 72.94, 68.84, 79.27 |
Refinement procedure
Resolution | 42.830 - 1.893 |
R-factor | 0.1873 |
Rwork | 0.186 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6te4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.573 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.830 | 1.960 |
High resolution limit [Å] | 1.893 | 1.893 |
Rmerge | 1.459 | |
Number of reflections | 94264 | 8315 |
<I/σ(I)> | 5.22 | |
Completeness [%] | 93.5 | |
Redundancy | 3.5 | |
CC(1/2) | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium acetate, 0.1 M Sodium cacodylate pH 6.5 and 18% PEG8,000 |