6YGV
tRNA-Guanine Transglycosylase (TGT) in co-crystallized complex with (E)-N-ethyl-4-oxo-4-phenylbut-2-enamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.824, 64.911, 71.070 |
Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
Resolution | 45.190 - 1.630 |
R-factor | 0.1325 |
Rwork | 0.131 |
R-free | 0.16430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.008 |
RMSD bond angle | 0.958 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.190 | 1.730 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 50766 | 8139 |
<I/σ(I)> | 25.55 | |
Completeness [%] | 99.5 | |
Redundancy | 6.8 | |
CC(1/2) | 1.000 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 100 mM TRIS pH 8.5, 20% (m/v) PEG3350 |