6YGQ
EclA N-terminal domain; PllA-like lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.793, 50.461, 73.859 |
| Unit cell angles | 90.00, 109.36, 90.00 |
Refinement procedure
| Resolution | 40.870 - 1.900 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ofz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.621 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.870 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 36259 | 2350 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.997 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.06 M divalents (magnesium chloride hexahydrate and calcium chloride dihydrate), 0.1 M Tris and bicine, pH 8.5, (40% v/v) glycerol, 20% w/v PEG 4000 |
