6YGL
TGT W326F mutant labelled mit 5F-Trp crystallised at pH 5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.130, 65.204, 70.673 |
Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
Resolution | 45.310 - 1.480 |
R-factor | 0.134 |
Rwork | 0.132 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.007 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.310 | 1.570 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 66504 | 10258 |
<I/σ(I)> | 15.06 | |
Completeness [%] | 97.4 | |
Redundancy | 2.8 | |
CC(1/2) | 0.999 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 100 mM MES pH 5.5, 0.5 mM DTT, 10% (v/v) DMSO, 13% (m/v) PEG8000 |