6YGB

Crystal structure of the NatC complex bound to CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	48.042, 139.789, 166.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.434 - 2.451
R-factor	0.2047
Rwork	0.203
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yga
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.434	45.434	2.600
High resolution limit [Å]	2.450	7.270	2.450
Rmerge	0.148	0.030	1.444
Rmeas	0.161	0.033	1.565
Total number of observations	278939
Number of reflections	42192	1787	6663
<I/σ(I)>	10.85	45.37	1.24
Completeness [%]	99.9	99.2	99.5
Redundancy	6.611	5.898	6.734
CC(1/2)	0.997	0.999	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	293	16.5% PEG 4000, 150 mM ammonium iodide and 100 mM sodium citrate, pH 6.3