6YG0
Crystal structure of S287D,T291D MKK7 (MAP2K7), apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.370, 69.790, 84.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.190 - 2.000 |
R-factor | 0.1968 |
Rwork | 0.194 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dyl |
RMSD bond length | 0.015 |
RMSD bond angle | 1.442 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.190 | 36.187 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.066 | 0.052 | 0.952 |
Rmeas | 0.071 | 0.057 | 1.030 |
Rpim | 0.027 | 0.023 | 0.387 |
Number of reflections | 24828 | 889 | 3550 |
<I/σ(I)> | 13.8 | 8.2 | 0.8 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 6.7 | 6.1 | 6.8 |
CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 277.15 | 16% PEG3350, 0.25 M ammonium acetate, 0.1 M tris, pH 8.2 |