6YFV

Crystal structure of Mtr4-Red1 minimal complex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2741
Spacegroup name	P 65 2 2
Unit cell lengths	172.186, 172.186, 145.078
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.706 - 2.750
R-factor	0.2174
Rwork	0.215
R-free	0.25730
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.341
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.710	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.129	3.048
Number of reflections	33467
<I/σ(I)>	25.61
Completeness [%]	100.0
Redundancy	39.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.1 M MES pH 6.5 30% PEG 300