Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 341.900, 341.900, 1253.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.955 - 3.800 |
| R-factor | 0.2199 |
| Rwork | 0.220 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.955 | 49.955 | 4.010 |
| High resolution limit [Å] | 3.780 | 11.080 | 3.780 |
| Rmerge | 0.597 | 0.137 | 2.764 |
| Rmeas | 0.613 | 0.141 | 2.837 |
| Total number of observations | 5672505 | ||
| Number of reflections | 275564 | 11506 | 40587 |
| <I/σ(I)> | 4.74 | 19.13 | 1 |
| Completeness [%] | 98.3 | 98.6 | 90 |
| Redundancy | 20.585 | 18.396 | 19.223 |
| CC(1/2) | 0.993 | 0.998 | 0.467 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% MPD, 0.02 M calcium chloride, 0.1 M sodium acetate pH 4.6 |
