Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 419.731, 419.731, 761.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.455 - 3.300 |
| R-factor | 0.2478 |
| Rwork | 0.248 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.455 | 97.455 | 3.480 |
| High resolution limit [Å] | 3.300 | 10.440 | 3.300 |
| Rmerge | 0.055 | 2.056 | |
| Rmeas | 0.469 | 0.063 | 2.349 |
| Rpim | 0.212 | 0.030 | 1.113 |
| Total number of observations | 1819911 | 58024 | 226817 |
| Number of reflections | 380562 | 12463 | 54974 |
| <I/σ(I)> | 4.3 | 17.8 | 0.7 |
| Completeness [%] | 99.5 | 99.4 | 98.9 |
| Redundancy | 4.8 | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 36% PEG 300, 0.05 M Bicine pH 9.0 |
