Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 269.417, 277.201, 277.444 |
| Unit cell angles | 103.91, 117.39, 106.96 |
Refinement procedure
| Resolution | 49.267 - 3.246 |
| R-factor | 0.2413 |
| Rwork | 0.241 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.267 | 49.267 | 3.440 |
| High resolution limit [Å] | 3.200 | 9.570 | 3.250 |
| Rmerge | 0.372 | 0.048 | 2.509 |
| Rmeas | 0.435 | 0.056 | 2.938 |
| Number of reflections | 911374 | 35312 | 137505 |
| <I/σ(I)> | 4.3 | 22.57 | 0.51 |
| Completeness [%] | 94.0 | 93.2 | 88 |
| Redundancy | 3.635 | 3.604 | 3.534 |
| CC(1/2) | 0.988 | 0.997 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 46% MPD, 0.1 M HEPES pH 7.5 |
