6YF5

Lamin A 17-70 coil1A dimer stabilized by C-terminal capping

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.980110
Spacegroup name	P 21 21 21
Unit cell lengths	27.374, 105.246, 119.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.220 - 1.830
R-factor	0.226
Rwork	0.225
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xa3
RMSD bond length	0.009
RMSD bond angle	1.471
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.050	1.860
High resolution limit [Å]	1.830	1.830
Number of reflections	31710	1487
<I/σ(I)>	9.73	1.08
Completeness [%]	100.0	98.54
Redundancy	10.22	7.6
CC(1/2)	0.999	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.9	277	35.7% (w/v) 1,6-hexanediol, 5% (w/v) PEG 1K and 0.1 M trisodium citrate dihydrate