6YEI

Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	93.843, 99.811, 317.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.260 - 2.020
R-factor	0.1607
Rwork	0.160
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b26
RMSD bond length	0.008
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.300	70.300	2.140
High resolution limit [Å]	2.020	5.940	2.020
Rmerge	0.071	0.027	0.804
Rpim	0.028	0.015	0.449
Number of reflections	164077	8204	8194
<I/σ(I)>	17.3	48.8	2.5
Completeness [%]	95.7	99.8	64.8
Redundancy	7.4	6.9	7.3
CC(1/2)	0.999	0.999	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	15% w/v PEG 6000, 100 mM MES pH 6.5, 10% (v/v) MPD