6YEG
Hybrid structure of the SPP1 tail tube by solid-state NMR and cryo EM - Final EM Refinement
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 900 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 4D HNhhNH |
| 10 | 1 | 2 | 3D HNhH |
| 11 | 1 | 3 | 3D HNhH |
| 12 | 1 | 7 | 3D HNhH |
| 13 | 1 | 6 | 3D HNhH |
| 14 | 1 | 5 | 3D HNhH |
| 15 | 1 | 4 | 3D HNhH |
| 16 | 1 | 8 | 3D HNhH |
| 17 | 1 | 9 | 3D HNhH |
| 18 | 1 | 10 | 3D HNhH |
| 2 | 1 | 2 | 3D HChH |
| 3 | 1 | 6 | 3D HChH |
| 4 | 1 | 2 | 2D hCH |
| 5 | 1 | 4 | 2D hCH |
| 6 | 1 | 6 | 2D hCH |
| 7 | 1 | 8 | 2D hCH |
| 8 | 1 | 10 | 2D hCH |
| 9 | 1 | 11 | 2D hCH |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7.4 | 1 | 291 |
Conformers
| Conformers Calculated Total Number | 1 |
| Conformers Submitted Total Number | 1 |
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4 |
| Software | RELION,PHENIX,EPU,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
