6YED

E.coli's Putrescine receptor PotF in its open apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	76.220, 53.450, 88.130
Unit cell angles	90.00, 111.59, 90.00

Refinement procedure

Resolution	45.900 - 2.180
R-factor	0.2303
Rwork	0.228
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.258
High resolution limit [Å]	2.180	2.180
Rmerge	0.157	0.970
Rmeas	0.164	1.023
Rpim	0.049	0.320
Number of reflections	67443	3433
<I/σ(I)>	13.39	4.65
Completeness [%]	100.0	99.91
Redundancy	10.9	9.7
CC(1/2)	0.997	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.1 M Tris pH 7.2, 0.2 M NaCl, 35% PEG 3000