6YEC

E.coli's Putrescine receptor PotF complexed with Spermine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	70.401, 70.401, 273.497
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.580 - 2.090
R-factor	0.2165
Rwork	0.215
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.001
RMSD bond angle	0.446
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.580	2.165
High resolution limit [Å]	2.090	2.090
Rmerge	0.108	3.334
Rmeas	0.114	3.490
Rpim	0.034	1.024
Number of reflections	47981	4689
<I/σ(I)>	14.62	0.65
Completeness [%]	100.0	100
Redundancy	11	11.4
CC(1/2)	0.999	0.229

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550