6YEC
E.coli's Putrescine receptor PotF complexed with Spermine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.401, 70.401, 273.497 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.580 - 2.090 |
R-factor | 0.2165 |
Rwork | 0.215 |
R-free | 0.24020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.446 |
Data reduction software | XDS (20180126) |
Data scaling software | XDS (20180126) |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.580 | 2.165 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.108 | 3.334 |
Rmeas | 0.114 | 3.490 |
Rpim | 0.034 | 1.024 |
Number of reflections | 47981 | 4689 |
<I/σ(I)> | 14.62 | 0.65 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 11.4 |
CC(1/2) | 0.999 | 0.229 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550 |