6YE8
E.coli's Putrescine receptor PotF complexed with Spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-13 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.830, 70.830, 272.083 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.710 - 1.500 |
R-factor | 0.1621 |
Rwork | 0.162 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.087 |
Data reduction software | XDS (20180126) |
Data scaling software | XDS (20180126) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.710 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.071 | 2.663 |
Rmeas | 0.075 | 2.794 |
Rpim | 0.022 | 0.838 |
Number of reflections | 128236 | 12611 |
<I/σ(I)> | 17.79 | 0.76 |
Completeness [%] | 100.0 | 99.99 |
Redundancy | 11.5 | 11 |
CC(1/2) | 1.000 | 0.264 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 10% Jeffamine M600 |