6YDL
Substrate-free beta-phosphoglucomutase from Lactococcus lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92819 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.280, 54.070, 81.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.650 - 1.520 |
R-factor | 0.18415 |
Rwork | 0.183 |
R-free | 0.21606 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2whe |
Data reduction software | XDS |
Data scaling software | xia2 (0.4.0.0) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.650 | 44.650 | 1.560 |
High resolution limit [Å] | 1.520 | 6.800 | 1.520 |
Rmerge | 0.044 | 0.017 | 1.190 |
Rmeas | 0.051 | 0.020 | 1.428 |
Rpim | 0.019 | 0.007 | 0.561 |
Number of reflections | 36815 | 486 | 2658 |
<I/σ(I)> | 18.8 | 71.6 | 1.3 |
Completeness [%] | 99.3 | 98.6 | 99.3 |
Redundancy | 7.2 | 6.4 | 6.3 |
CC(1/2) | 1.000 | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | PEG 4000, sodium acetate (200 mM), tris (100 mM), HEPES (50 mM), magnesium chloride (5 mM), EDTA (1 mM), sodium azide (2 mM), beta-phosphoglucomutase (0.6 mM) |