6YDC
X-ray structure of LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.790, 120.800, 136.360 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.21695 |
| Rwork | 0.215 |
| R-free | 0.26209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ach |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (v5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 85419 | 6184 |
| <I/σ(I)> | 5.54 | 1.44 |
| Completeness [%] | 98.4 | 97.2 |
| Redundancy | 4.55 | 4.16 |
| CC(1/2) | 0.989 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 0.1 M NaCl, 0.1 M HEPES pH 6.5, 1.5 M (NH4)2SO4 |
