6YCZ

Plasmodium falciparum Myosin A delta-Nter, Post-Rigor state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	P 21 21 21
Unit cell lengths	90.080, 114.430, 170.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.300 - 3.270
R-factor	0.182
Rwork	0.179
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i7d
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	3.387
High resolution limit [Å]	3.270	3.270
Number of reflections	27977	2748
<I/σ(I)>	9.02
Completeness [%]	99.9
Redundancy	12.8
CC(1/2)	0.992	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2.0M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 6% PEG400