6YCX

Plasmodium falciparum Myosin A full-length, pre-powerstroke state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978570
Spacegroup name	P 21 21 2
Unit cell lengths	168.240, 287.430, 78.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.400 - 3.990
R-factor	0.239
Rwork	0.237
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i7e
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	4.133
High resolution limit [Å]	3.990	3.990
Rmerge	0.444
Number of reflections	33243	3194
<I/σ(I)>	4.19
Completeness [%]	99.5
Redundancy	10.3
CC(1/2)	0.989	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290.15	1M Na Citrate tribasic; 0.01M Na Borate pH 8.0