6YC7

Structure of adenylylated C. glutamicum GlnK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-19
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 32
Unit cell lengths	53.444, 53.444, 179.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.970 - 1.800
R-factor	0.1888
Rwork	0.188
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cy6
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.970	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.058	1.079
Number of reflections	53142	5326
<I/σ(I)>	17.54
Completeness [%]	99.6
Redundancy	5.1
CC(1/2)	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	0.06 M MgCl2, 0.06 M CaCl2, 0:1M MOPS pH 7.1, 0.1M HEPES pH 7.1, 15% (v/v) MPD, 15% (w/v) PEG 1000 and 15% (w/v) PEG 3350