6YC3
Crystal structure of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Replaces: 6REWExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 131.866, 240.323, 135.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1697 |
Rwork | 0.168 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rew |
RMSD bond length | 0.003 |
RMSD bond angle | 1.071 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.160 | 48.160 | 2.030 |
High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
Rmerge | 0.076 | 0.023 | 1.749 |
Rmeas | 0.082 | 0.025 | 1.876 |
Rpim | 0.030 | 0.009 | 0.674 |
Total number of observations | 1101707 | 6427 | 54868 |
Number of reflections | 144508 | 956 | 7135 |
<I/σ(I)> | 13.2 | 47.6 | 1.2 |
Completeness [%] | 99.9 | 97.1 | 100 |
Redundancy | 7.6 | 6.7 | 7.7 |
CC(1/2) | 0.999 | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8 | 293 | 1.2 M NaMal pH 8.0 |