6YBR
RT structure of Glucose Isomerase obtained at 1.20 A resolution from crystal grown in a Mylar microchip.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 293.5 |
Detector technology | PIXEL |
Collection date | 2017-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 93.990, 99.380, 103.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.990 - 1.200 |
R-factor | 0.1215 |
Rwork | 0.121 |
R-free | 0.13050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a8i |
RMSD bond length | 0.005 |
RMSD bond angle | 0.889 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.990 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 149874 | 14856 |
<I/σ(I)> | 17 | |
Completeness [%] | 100.0 | |
Redundancy | 8.86 | |
CC(1/2) | 0.994 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 7 | 293.5 | 0.1 M MgCl2, 10% w/v PEG 1K, 0.1 M Hepes pH 7.0 |