6YBO
RT structure of Glucose Isomerase obtained at 1.06 A resolution from crystal grown in a Kapton microchip.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 293.5 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.940, 99.370, 103.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.120 - 1.060 |
| R-factor | 0.1233 |
| Rwork | 0.123 |
| R-free | 0.13100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a8i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.120 | 1.090 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Number of reflections | 200000 | 8688 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 92.3 | 40.2 |
| Redundancy | 7.93 | |
| CC(1/2) | 0.987 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7 | 293.5 | 0.1 M MgCl2, 10% w/v PEG 1K, 0.1 M Hepes pH 7.0 |
