6YBE
RNASE 3/1 version2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792568 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 43.210, 61.770, 51.346 |
| Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
| Resolution | 38.450 - 1.140 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.16550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2k11 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.450 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Number of reflections | 43663 | 2247 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 92.6 | 90.9 |
| Redundancy | 2.1 | |
| CC(1/2) | 0.996 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium acetate pH 5 and 1 M sodium chloride |
