6YB3
Crystal structure of a native BcsRQ complex purified and crystallized in the absence of nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.000, 78.990, 66.820 |
| Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
| Resolution | 46.670 - 1.590 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yar |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17rc2_3619) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 74677 | 10322 |
| <I/σ(I)> | 11.46 | 0.87 |
| Completeness [%] | 95.7 | 82.4 |
| Redundancy | 3.84 | 2.7 |
| CC(1/2) | 0.998 | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 15-22% PEG 2000, 100 mM MES or Bis-Tris with pH 5.5-6.5 and 0-7% glycerol or xylitol |
