6YB2

Crystal structure of a parallel hexameric coiled coil CC-Type2-(TaId)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-05-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.94999
Spacegroup name	P 21 21 21
Unit cell lengths	31.010, 55.990, 90.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.817 - 1.180
R-factor	0.1609
Rwork	0.159
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Parametric Model
RMSD bond length	0.008
RMSD bond angle	0.876
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.817	1.200
High resolution limit [Å]	1.180	1.180
Rmerge	0.071	1.690
Number of reflections	52714	21633
<I/σ(I)>	12.8	1.1
Completeness [%]	99.8	98.8
Redundancy	11.4
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris, 40% v/v MPD