6YB2
Crystal structure of a parallel hexameric coiled coil CC-Type2-(TaId)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-05-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.94999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.010, 55.990, 90.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.817 - 1.180 |
R-factor | 0.1609 |
Rwork | 0.159 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Parametric Model |
RMSD bond length | 0.008 |
RMSD bond angle | 0.876 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.817 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.071 | 1.690 |
Number of reflections | 52714 | 21633 |
<I/σ(I)> | 12.8 | 1.1 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 11.4 | |
CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris, 40% v/v MPD |