6YB1

Crystal structure of an antiparallel octameric transmembrane coiled coil K2-CCTM-VbIc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11582
Spacegroup name	P 31 1 2
Unit cell lengths	47.864, 47.864, 103.153
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.380 - 2.150
R-factor	0.1817
Rwork	0.180
R-free	0.20950
Structure solution method	AB INITIO PHASING
RMSD bond length	0.004
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.500	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.099	1.275
Number of reflections	7587	702
<I/σ(I)>	22.1	1.84
Completeness [%]	99.2	99.2
Redundancy	19	13.7
CC(1/2)	1.000	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	10	293	Monoolein lipidic cubic phase, 0.05 M Glycine, 55% v/v PEG400