6YB0

Crystal structure of a parallel hexameric coiled coil CC-Type2-(TaSd)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.91974
Spacegroup name	P 21 21 21
Unit cell lengths	76.404, 81.566, 90.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.630 - 1.860
R-factor	0.1912
Rwork	0.190
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Parametric Model
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.600	1.890
High resolution limit [Å]	1.860	1.860
Rmerge	0.121	2.370
Number of reflections	48308	2272
<I/σ(I)>	10.6	1.07
Completeness [%]	99.9	95.6
Redundancy	12.9
CC(1/2)	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.5 M NaCl, 0.05 M BIS-Tris