6YAZ

Crystal structure of a parallel hexameric coiled coil CC-Type2-(TaId)5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	40.136, 43.307, 83.024
Unit cell angles	101.53, 97.69, 90.21

Refinement procedure

Resolution	40.290 - 1.940
R-factor	0.2115
Rwork	0.210
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Parametric Model
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	1.970
High resolution limit [Å]	1.940	1.940
Rmerge	0.102
Number of reflections	39971	1922
<I/σ(I)>	7.7	1.1
Completeness [%]	99.2	94.4
Redundancy	5.9
CC(1/2)	0.993	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M NaNO3, 0.05 M Bis-Tris propane, 10% PEG3350