6YAY
Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-11 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.060, 78.780, 66.600 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 46.640 - 2.090 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.22310 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.498 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17rc2_3619) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 66678 | 10300 |
<I/σ(I)> | 9.56 | 1.15 |
Completeness [%] | 98.4 | 94 |
Redundancy | 3.9 | 3.52 |
CC(1/2) | 0.998 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 2000, MES pH6.5, glycerol |