6YAR
Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of AppCp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.120, 78.430, 65.220 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 45.160 - 1.900 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21760 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.784 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17rc2_3619) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 81355 | 10020 |
| <I/σ(I)> | 10.34 | 0.99 |
| Completeness [%] | 93.3 | 71 |
| Redundancy | 5.16 | 3.6 |
| CC(1/2) | 0.999 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 2000, MES pH6.5, glycerol |
