6YAK
Split gene transketolase, active alpha2beta2 heterotetramer
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 123.078, 130.047, 165.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.870 - 1.340 |
| Rwork | 0.141 |
| R-free | 0.15880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.878 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmeas | 0.056 | 1.619 |
| Number of reflections | 288149 | 11686 |
| <I/σ(I)> | 16.4 | 0.8 |
| Completeness [%] | 97.7 | 81.3 |
| Redundancy | 6.1 | 3.2 |
| CC(1/2) | 1.000 | 0.266 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | 2.1 M DL-malic acid pH 7.0 |
