6YA2

Crystal structure of TSWV glycoprotein N ectodomain (Trypsin treated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	69.660, 76.010, 71.180
Unit cell angles	90.00, 106.56, 90.00

Refinement procedure

Resolution	18.850 - 2.500
R-factor	0.2145
Rwork	0.213
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ya0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.850	18.850	2.600
High resolution limit [Å]	2.500	15.000	2.500
Rmerge	0.100	0.040	1.833
Rmeas	0.108	0.044	1.977
Total number of observations	170741
Number of reflections	24477	62	2700
<I/σ(I)>	13.14	40.11	1
Completeness [%]	98.5	48.4	98.2
Redundancy	6.976	5.161	7.12
CC(1/2)	0.999	0.999	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	19% PEG 8,000 (w/v) 0.1 M tris pH 7.5 0.2 M magnesium chloride hexahydrate