6YA0

Crystal structure of TSWV glycoprotein N ectodomain (Trypsin treated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	68.820, 74.850, 81.280
Unit cell angles	90.00, 103.27, 90.00

Refinement procedure

Resolution	49.910 - 2.860
R-factor	0.2219
Rwork	0.217
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y9m
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.910	49.910	2.930
High resolution limit [Å]	2.860	12.790	2.860
Rmerge	0.064	0.031	1.635
Rmeas	0.076	0.037	1.926
Total number of observations	63929
Number of reflections	18724	224	1401
<I/σ(I)>	10.5	31.45	0.88
Completeness [%]	99.6	97.4	99.9
Redundancy	3.414	3.25	3.562
CC(1/2)	0.998	0.998	0.437

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	28% PEG 3350 (w/v) 0.1 M tris pH 7.5 2% Ethanol