6Y9M

Crystal structure of TSWV glycoprotein N ectodomain (sGn)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03961
Spacegroup name	C 2 2 21
Unit cell lengths	167.200, 218.200, 145.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.160 - 3.400
R-factor	0.2605
Rwork	0.259
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y9l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.160	49.160	3.450
High resolution limit [Å]	3.400	20.000	3.400
Rmerge	0.143	0.057	7.816
Rmeas	0.145	0.058	7.951
Total number of observations	1462177
Number of reflections	36920	198	1543
<I/σ(I)>	20.04	67.85	0.6
Completeness [%]	99.7	91.2	97.8
Redundancy	39.604	31.157	29.657
CC(1/2)	1.000	0.999	0.432

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	25% PEG 4000 0.1M Tris pH 8.5