6Y9L

Crystal structure of TSWV glycoprotein N ectodomain (sGn)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	165.992, 215.878, 146.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.020 - 4.100
R-factor	0.2784
Rwork	0.277
R-free	0.30190
Structure solution method	MIRAS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.020	49.020	4.210
High resolution limit [Å]	4.100	18.360	4.100
Rmerge	0.104	0.047	3.877
Rmeas	0.114	0.052	4.237
Total number of observations	133260
Number of reflections	20685	252	1288
<I/σ(I)>	8.39	29.49	0.41
Completeness [%]	98.8	92.3	85.4
Redundancy	6.442	5.048	5.871
CC(1/2)	0.998	0.998	0.274

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1.8 M ammonium sulfate 0.1 M Tris pH 8.5