6Y9J
Crystal Structure of subtype-switched Epithelial Adhesin 1 to 9 A domain (Epa1-CBL2Epa9) from Candida glabrata in complex with beta-lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.220, 104.140, 69.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.545 - 1.100 |
| R-factor | 0.1468 |
| Rwork | 0.146 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4asl |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.545 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.054 | 1.449 |
| Number of reflections | 107346 | 4801 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.1 | 90.4 |
| Redundancy | 6.8 | 3.4 |
| CC(1/2) | 0.999 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M MES pH 6.5, 0.2 M ammonium sulfate, 25 % PEG 5000 MME |
